Isolation and partial chemical characterization of a 64,000-dalton glycoprotein of human cytomegalovirus.
نویسندگان
چکیده
A guanidinium chloride extract of [3H]glucosamine- and [35S]methionine-labeled virions plus dense bodies of human cytomegalovirus (Towne) was separated by reverse-phase high-pressure liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate revealed the major peak to be a glycoprotein with a relative mass of 64,000. This glycoprotein (HCMVgp64) was characterized by amino acid analysis and a high-pressure liquid chromatographic map of its tryptic peptides.
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ورودعنوان ژورنال:
- Journal of virology
دوره 49 1 شماره
صفحات -
تاریخ انتشار 1984